「0.0375M KC1」でググってみる。

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マスコミが大騒ぎでしたよね。
で。山中教授が弁明しなければいけなくなりましたよね。

TVでの続報はどうなっていることやら。
ということで、ググってみました。
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>「0.0375M KC1」でググると二番目あたりに面白いものが出てくるね。

それから。
この方のことも気になったので、併せてググってみました。
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博多駅に向かって3週間が経った人はいずこ?
http://anond.hatelabo.jp/20140225162613



1. Rat Spleens from 6 month rats, LSM separated lymphocytes, treated exactly as published, cultured in DMEM/F12,2%B27,Lif. Oct4 detected after 7 days by western Blot.
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Multicolor karyotype analyses of mouse embryonic stem cells (Guo et.al., 2005)

colcemid (final concentration 0.270 μg/ml) to the culture medium for
2.5 h at 37 ~ C in 5% CO2. Cells were washed with PBS, treated with trypsin-
ethylenediaminetetraacetic acid (EDTA), resuspended into cell medium and
centrifuged for 5 min at 1200 rpm. To the cell pellet in 3 ml of PBS, 7 ml
of a prewarmed hypotonic 0.0375 M KC1 solution was added. Cells were
incubated for 20 min at 37 °C. Cells were centrifuged for 5 min at 1200 rpm
and the pellet was resuspended in 3-5 ml of 0.0375 M KC1 solution. The
cells were fixed with methanol/acetic acid (3:1, vohvol) by gently pipetting.
Fixation was performed four times


OCRで読み取ったまま、・・・。
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http://patentscope.wipo.int/search/en/WO2013163296
[00290] Karyotype analysis. Karyotype analysis was performed by Multicolor FISH analysis (M-FISH). Subconfluent STAPS cells were arrested in metaphase by colcemid (final concentration 0.270 μg/ml) to the culture medium for 2.5 h at 37°C in 5% C02.
Cells were washed with PBS, treated with trypsin/ethylenediaminetetraacetic acid (EDTA), resuspended into cell medium and centrifuged for 5 min at 1200 rpm.
To the cell pellet in 3 ml of PBS, 7 ml of a prewarmed hypotonic 0.0375 M 【KC 1】 solution was added.
Cells were incubated for 20 min at 37°C. Cells were centrifuged for 5 min at 1200 rpm and the pellet was resuspended in 3-5 ml of 0.0375 M 【KC1】 solution.
The cells were fixed with methanol/acetic acid (3: 1; vol/vol) by gently pipetting. Fixation was performed four times prior to spreading the cells on glass slides.



http://stapcells.blogspot.jp/2014/02/blog-post_7834.html

こちらの翻訳されたものがオススメかと思います。
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STAP細胞ちょっと話が上手すぎる?
http://www.myscizzle.com/blog/stapcells-japanese/


やっぱり・・・⁇
再現性の有無待ちですか。
どうなりますことやら。



↓励みになります♪1日1回のクリックご協力をお願いいたします。
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[PR]
by Lisa_cerise | 2014-03-01 00:50 | つぶやき_STAP細胞

日本人の心を大切に          ・・・Lisa


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